5E5W

Hemagglutinin-esterase-fusion mutant structure of influenza D virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	164.335, 164.335, 164.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 2.400
R-factor	0.207
Rwork	0.205
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1flc
RMSD bond length	0.018
RMSD bond angle	1.488
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.121	0.769
Number of reflections	57383
<I/σ(I)>	2.6	2.6
Completeness [%]	100.0	100
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M PCTP (Propionic acid, Cacodylate, Bis-tris propane system) buffer pH 8.5, 22.5%(w/v) PEG1500