5E4W
Crystal structure of cpSRP43 chromodomains 2 and 3 in complex with the Alb3 tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.592, 163.668, 37.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.800 |
| R-factor | 0.2517 |
| Rwork | 0.249 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dxb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.240 | 0.930 |
| Number of reflections | 12743 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 3350, 0.2 M Ca(OAc)2 |
