5E38

Structural basis of mapping the spontaneous mutations with 5-flourouracil in uracil phosphoribosyltransferase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-10
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 32
Unit cell lengths	118.089, 118.089, 77.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.224 - 3.000
R-factor	0.2112
Rwork	0.205
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o5o
RMSD bond length	0.010
RMSD bond angle	1.387
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	22764
<I/σ(I)>	7.8	3.95
Completeness [%]	97.1	99.3
Redundancy	6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Sodium formate