5E2W
Anti-TAU AT8 FAB with triply phosphorylated TAU peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-29 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.620, 61.020, 84.120 |
| Unit cell angles | 90.00, 133.11, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.500 |
| R-factor | 0.21598 |
| Rwork | 0.216 |
| R-free | 0.21998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e2v |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.529 |
| Number of reflections | 66342 | |
| <I/σ(I)> | 19.1 | 3.6 |
| Completeness [%] | 98.1 | 97.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, PH 7.5, 18% PEG 3350, 0.2 M NA FORMATE, MOTHER LIQUOR + 24% GLYCEROL |
