5E0V
Human PCNA variant (S228I) complexed with FEN1 at 2.1 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 3 |
| Unit cell lengths | 80.721, 80.721, 66.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.945 - 2.074 |
| R-factor | 0.2155 |
| Rwork | 0.202 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ul7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.950 | 2.120 |
| High resolution limit [Å] | 2.074 | 2.074 |
| Rmerge | 0.078 | 0.636 |
| Number of reflections | 29298 | |
| <I/σ(I)> | 16.68 | 2.1 |
| Completeness [%] | 99.7 | 98.33 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.35 M ammonium sulfate, 100 mM sodium acetate pH 4.6, 40% (v/v) -1,3-Butanediol |
