5E0T
Human PCNA mutant - S228I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 162.324, 162.324, 139.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.854 - 2.665 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vym |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.629 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.860 | 2.720 |
| High resolution limit [Å] | 2.665 | 2.665 |
| Rmerge | 0.851 | |
| Number of reflections | 53711 | |
| <I/σ(I)> | 12.3 | 2.28 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM sodium acetate, pH4.5, 2M ammonium sulfate |
