5DY9

Y68T Hfq from Methanococcus jannaschii in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 1 21 1
Unit cell lengths	60.636, 67.635, 91.165
Unit cell angles	90.00, 90.95, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.1697
Rwork	0.169
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge		0.683
Number of reflections	96577
<I/σ(I)>	18.17	2.28
Completeness [%]	99.2	98
Redundancy	4.5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	50% PEG200, 100 mM Tris-HCl, pH 8.0 (JBScreen Nuc-Pro 1)