5DX5
Crystal structure of methionine gamma-lyase from Clostridium sporogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.090, 91.090, 175.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.370 |
R-factor | 0.1646 |
Rwork | 0.162 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.027 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.450 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.097 | 0.620 |
Number of reflections | 57232 | |
<I/σ(I)> | 17 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.9 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | MGL 20mg/ml, PEG MME 2000 35%, 50 ml TrisHCl (pH=8.5), 0.2 mM PLP |