5DV7

Crystal Structure of NF90 tandem dsRBDs with dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.17
Spacegroup name	P 31 1 2
Unit cell lengths	100.104, 100.104, 108.007
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.413 - 3.500
R-factor	0.2405
Rwork	0.239
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1x 2dmy
RMSD bond length	0.010
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.086	0.912
Number of reflections	7981
<I/σ(I)>	22.1	3.6
Completeness [%]	99.9	99.9
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	50mM sodium citrate pH 5.0, 1.0M ammonium formate