5DSV
Crystal structure of human proteasome alpha7 tetradecamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 132.481, 132.481, 444.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.750 |
| R-factor | 0.21349 |
| Rwork | 0.209 |
| R-free | 0.29107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j2p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | SCALEPACK |
| Data scaling software | DENZO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.810 |
| High resolution limit [Å] | 3.750 | 3.750 |
| Rmerge | 0.081 | 0.497 |
| Number of reflections | 40504 | |
| <I/σ(I)> | 16.3 | 2.5 |
| Completeness [%] | 97.1 | 98.5 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 24% PEG400, 100 mM Tris-HCl (pH 7.5), 0.2 M magnesium chloride |
