5DSE

Crystal Structure of the TTC7B/Hyccin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.779, 168.068, 239.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.959 - 2.900
R-factor	0.2132
Rwork	0.212
R-free	0.24170
RMSD bond length	0.003
RMSD bond angle	0.660
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: dev_1630))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.960	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	64827
<I/σ(I)>	22.8
Completeness [%]	99.5	98.9
Redundancy	6.6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.35 ul protein + 1.35 ul mother liquor (0.1 M HEPES [pH 7.0-7.4], 4-5% polyethylene glycol [PEG] 8,000) + 0.3 ul 0.2 M 3-(1-Pyridino)-1-propane sulfonate (NDSB-201)