5DS5
Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-07-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.656, 165.928, 167.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.475 - 2.951 |
| R-factor | 0.2348 |
| Rwork | 0.234 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p6i |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.718 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.475 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.108 | 0.863 |
| Number of reflections | 44960 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 50 mM MES, pH 6.1, 10% isopropanol and 20 mM MgCl2 |
