5DRZ
Crystal structure of anti-HIV-1 antibody F240 Fab in complex with gp41 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.317, 60.488, 169.228 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 168.790 - 2.540 |
| R-factor | 0.20649 |
| Rwork | 0.204 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tnn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 168.790 | 2.580 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.158 | 0.957 |
| Number of reflections | 33289 | |
| <I/σ(I)> | 9.4 | 1.4 |
| Completeness [%] | 98.8 | 98.6 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 25% PEG 3350, 15% Isopropanol, 0.2M Ammonium citrate pH4.5 |
