5DR5
Crystal structure of the sclerostin-neutralizing Fab AbD09097
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.193, 78.495, 59.196 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 20.770 - 1.850 |
| R-factor | 0.1913 |
| Rwork | 0.188 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nh7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.006 |
| Data reduction software | CrystalClear (9.2SSI) |
| Data scaling software | CrystalClear (9.2SSI) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.770 | 20.770 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.980 | 1.850 |
| Rmerge | 0.068 | 0.034 | 0.300 |
| Total number of observations | 126958 | 13809 | 8261 |
| Number of reflections | 34389 | ||
| <I/σ(I)> | 10.5 | 28.1 | 3.7 |
| Completeness [%] | 97.9 | 99.7 | 80.1 |
| Redundancy | 3.66 | 3.81 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 20% (w/v) PEG3350, 0.1M HEPES pH 7.5, 10mM zinc chloride |
