5DQ0
Structure of human neuropilin-2 b1 domain with novel and unique zinc binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.390, 105.270, 45.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.130 - 1.800 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qqj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.753 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.130 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.230 |
| Number of reflections | 22736 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 25 % PEG 550 MME, 0.1 M MES pH 6.5, 0.01 M ZnSO4 |
