5DOB

Crystal structure of the Human Cytomegalovirus Nuclear Egress Complex (NEC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	142.254, 33.978, 92.995
Unit cell angles	90.00, 121.76, 90.00

Refinement procedure

Resolution	79.070 - 2.470
R-factor	0.25644
Rwork	0.254
R-free	0.29920
RMSD bond length	0.005
RMSD bond angle	1.019
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.080
High resolution limit [Å]	2.470	2.470
Number of reflections	13720
<I/σ(I)>	14.47
Completeness [%]	97.5	88.9
Redundancy	3.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	298	0.1 M Tris, 0.1 M NaCl, 10-15% PEG 3350 (w/v), 0.3-0.5 M CaCl2