5DNF
Crystal structure of CC chemokine 5 (CCL5) oligomer in complex with heparin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.722 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 121.190, 210.063, 123.827 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.695 - 2.549 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23370 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.735 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.695 | 2.590 |
| High resolution limit [Å] | 2.549 | 2.550 |
| Rmerge | 0.084 | 0.750 |
| Number of reflections | 51550 | |
| <I/σ(I)> | 54 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 64.8 | 52.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303.15 | 0.1M Tris, pH 7.5; 1.8 M (NH4)2SO4 |
