5DLQ
Crystal structure of RanGTP-Exportin 4-eIF5A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.616, 98.616, 726.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.407 - 3.200 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.26950 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.407 | 3.080 | |
| High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
| Rmerge | 0.087 | 0.014 | 1.612 |
| Rmeas | 0.097 | 0.015 | 1.800 |
| Total number of observations | 828210 | ||
| Number of reflections | 157964 | 1759 | 11558 |
| <I/σ(I)> | 14.55 | 79.03 | 0.99 |
| Completeness [%] | 99.9 | 98.3 | 99 |
| Redundancy | 9.9 | 10.2 | |
| CC(1/2) | 0.999 | 1.000 | 0.455 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.26 | 293 | Peg 400, MES |
| 2 | VAPOR DIFFUSION, HANGING DROP | 6.26 | 293 | Peg 400, MES, DTT |
