5DLQ

Crystal structure of RanGTP-Exportin 4-eIF5A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	98.616, 98.616, 726.864
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.407 - 3.200
R-factor	0.2235
Rwork	0.221
R-free	0.26950
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.407		3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.087	0.014	1.612
Rmeas	0.097	0.015	1.800
Total number of observations	828210
Number of reflections	157964	1759	11558
<I/σ(I)>	14.55	79.03	0.99
Completeness [%]	99.9	98.3	99
Redundancy	9.9		10.2
CC(1/2)	0.999	1.000	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.26	293	Peg 400, MES
2	VAPOR DIFFUSION, HANGING DROP	6.26	293	Peg 400, MES, DTT