5DKA
A C2HC zinc finger is essential for the activity of the RING ubiquitin ligase RNF125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.670, 52.320, 73.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.710 - 1.550 |
| R-factor | 0.2113 |
| Rwork | 0.210 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hcs |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.142 |
| Data reduction software | XDS (November 3, 2014) |
| Data scaling software | XSCALE (November 3, 2014) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.055 | 0.799 |
| Number of reflections | 28019 | |
| <I/σ(I)> | 10.2 | 1.3 |
| Completeness [%] | 94.0 | 94.7 |
| Redundancy | 2.9 | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 273 | 0.1 M HEPES, 10% PEG 8K |
