5DJD
Fc Heterodimer Design 5.1 T366V + Y407F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2013-10-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.385, 67.435, 79.029 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.22264 |
| Rwork | 0.220 |
| R-free | 0.27571 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 67.435 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.071 | 0.508 | |
| Rmeas | 0.170 | ||
| Rpim | 0.088 | 0.043 | 0.332 |
| Total number of observations | 99778 | 2830 | 14869 |
| Number of reflections | 26867 | ||
| <I/σ(I)> | 5.1 | 8.6 | 2 |
| Completeness [%] | 97.9 | 90.1 | 99.9 |
| Redundancy | 3.7 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Imidazole pH 7 + 20% PEG 6000 |
