5DJ8

Fc Heterodimer Design 7.7 D399M/Y407A + T366V/K409I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2013-10-02
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	60.508, 67.669, 78.376
Unit cell angles	90.00, 104.34, 90.00

Refinement procedure

Resolution	28.800 - 2.400
R-factor	0.21674
Rwork	0.214
R-free	0.26025
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.518
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.800	67.669	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.047	0.455
Rmeas	0.121
Rpim	0.064	0.030	0.315
Total number of observations	82649	2636	10554
Number of reflections	23694
<I/σ(I)>	7.3	14.2	2.4
Completeness [%]	98.0	94.2	96.4
Redundancy	3.5	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	31% PEG 3350 + 100mM Ammonium Iodide