5DIS

Crystal structure of a CRM1-RanGTP-SPN1 export complex bound to a 113 amino acid FG-repeat containing fragment of Nup214

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	112.330, 248.970, 210.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.485 - 2.850
R-factor	0.2078
Rwork	0.206
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gjx 1anf
RMSD bond length	0.006
RMSD bond angle	0.925
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.490		2.950
High resolution limit [Å]	2.850	50.000	2.850
Rmerge	0.055		0.571
Rmeas	0.062		0.644
Total number of observations	313050
Number of reflections	67922		6140
<I/σ(I)>	15.52		2.01
Completeness [%]	98.2		91.4
Redundancy	4.6		4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	5% PEG 8000, 0.2M L-proline, 0.1M Tris pH 7.5, 4 mM D-maltose, 180 mM LiCl