5DI8
Fc Knob-Hole Heterodimer T366W + T366S/L368A/Y407V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2013-05-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.297, 66.835, 79.214 |
| Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.22666 |
| Rwork | 0.224 |
| R-free | 0.27511 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dn2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.551 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 66.835 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.045 | 0.350 | |
| Rmeas | 0.088 | ||
| Rpim | 0.045 | 0.027 | 0.248 |
| Total number of observations | 173293 | 4765 | 24277 |
| Number of reflections | 46181 | ||
| <I/σ(I)> | 9.5 | 16.1 | 3 |
| Completeness [%] | 96.2 | 86 | 95.4 |
| Redundancy | 3.8 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Hepes pH 7.5 + 2% Isopropanol + 18% PEG 8K + 100mM Sodium Acetate |
