5DI0
Crystal structure of Dln1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.111, 95.100, 147.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.600 - 1.700 |
| R-factor | 0.17407 |
| Rwork | 0.172 |
| R-free | 0.20887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zno |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.459 | |
| Number of reflections | 87589 | |
| <I/σ(I)> | 14.8 | 2.9 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 289 | 12% PEG 4K,0.2M NH4Ac, 0.1M NaAc pH 4.0 |
