5DFR

CRYSTAL STRUCTURE OF UNLIGANDED ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. LIGAND-INDUCED CONFORMATIONAL CHANGES AND COOPERATIVITY IN BINDING

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	68.730, 68.730, 83.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.198
RMSD bond length	0.026
RMSD bond angle	0.066
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	2.300 *
High resolution limit [Å]	2.050 *
Rmerge	0.043 *
Total number of observations	14730 *
Number of reflections	14169 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)	unliganded
2	1	drop	sodium/potassium phthalate	50 (mM)
3	1	drop	PEG6000	50 (%(w/w))
4	1	reservoir	PEG6000	25 (%)