5DFH
Human APE1 mismatch product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.434, 61.603, 72.277 |
| Unit cell angles | 83.86, 78.76, 88.19 |
Refinement procedure
| Resolution | 26.260 - 1.949 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iem |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.949 |
| Rmerge | 0.058 |
| <I/σ(I)> | 20.8 |
| Completeness [%] | 96.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 10% Peg20000, 100 mM sodium citrate, 200 mM MgCl |
