5DFF

Human APE1 product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-22
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	44.514, 61.716, 72.053
Unit cell angles	83.89, 78.81, 88.00

Refinement procedure

Resolution	24.377 - 1.570
R-factor	0.1874
Rwork	0.187
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.079
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.570	1.570
Rmerge	0.115	0.570
Number of reflections	103855
<I/σ(I)>	16.37	2.1
Completeness [%]	99.6	94.8
Redundancy	4.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2