5DEP

Structure of Pseudomonas aeruginosa LpxA in complex with UDP-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1270
Spacegroup name	P 21 21 21
Unit cell lengths	80.038, 83.559, 222.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.210 - 2.160
R-factor	0.18
Rwork	0.177
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.558
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall
Low resolution limit [Å]	111.210
High resolution limit [Å]	2.060
Number of reflections	79955
<I/σ(I)>	13.128
Completeness [%]	98.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M calcium acetate, 0.1M imidazole pH8.0, 20% PEG 1000, 50mM UDP-GlcNAc