5DE5
Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-23 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.1053 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.060, 130.010, 36.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.218 - 3.001 |
R-factor | 0.1958 |
Rwork | 0.195 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.846 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.011 | 0.665 |
Number of reflections | 10504 | |
<I/σ(I)> | 12.9 | 3.3 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 7.4 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M Na-citrate pH 5.6, 0.2 M ammonium acetate, 18% PEG 4000 |