5DBR

Ca2+ CaM with human cardiac Na+ channel (NaV1.5) inactivation gate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-31
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	58.086, 58.086, 102.638
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.300 - 2.250
R-factor	0.2236
Rwork	0.220
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4djc
RMSD bond length	0.018
RMSD bond angle	1.913
Data reduction software	PROTEUM PLUS
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.300	2.300
High resolution limit [Å]	2.250	2.250
Rmerge	0.090	0.446
Number of reflections	9401
<I/σ(I)>	18.4	4.1
Completeness [%]	99.6	100
Redundancy	15.6	10.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.2M sodium malonate pH 4.0, 20% w/v PEG 3350