5DB6
Structure of human DNA polymerase beta Host-Guest complex with the N7MG base paired with a dC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.138, 78.828, 54.764 |
| Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
| Resolution | 19.707 - 2.830 |
| R-factor | 0.1889 |
| Rwork | 0.185 |
| R-free | 0.26590 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.834 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.830 |
| Number of reflections | 10562 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 100.0 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole |
