5DAR
CRYSTAL STRUCTURE OF THE BASE OF THE RIBOSOMAL P STALK FROM METHANOCOCCUS JANNASCHII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-07-22 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.396, 88.451, 95.231 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.26607 |
| Rwork | 0.264 |
| R-free | 0.29679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5col 5d6g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.672 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.500 | |
| Number of reflections | 25542 | |
| <I/σ(I)> | 7.01 | 2.09 |
| Completeness [%] | 97.4 | 96.9 |
| Redundancy | 4.35 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 50 mM Tris-HCl, pH 7.5, 0.15 M KCl, 20 mM MgCl2, 15% PEG 4000 |
