5D9Y

Crystal structure of TET2-5fC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	48.154, 87.958, 268.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.767 - 1.971
R-factor	0.2074
Rwork	0.205
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm6
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.040
High resolution limit [Å]	1.970	4.240	1.970
Rmerge	0.055	0.036	0.624
Rmeas	0.060	0.039	0.695
Rpim	0.024	0.016	0.299
Total number of observations	242789
Number of reflections	57919
<I/σ(I)>	7.5
Completeness [%]	98.9	99.8	93.2
Redundancy	6	6.3	4.7
CC(1/2)		0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	277	21% PEGMME2000, 0.1M MES