5D9I
SV40 Large T antigen origin binding domain bound to artificial DNA fork
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.192, 63.393, 64.891 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.18174 |
| Rwork | 0.180 |
| R-free | 0.22257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fuf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.709 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0129) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 37425 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 88.8 | 54.7 |
| Redundancy | 7.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris, 300 mM NH4Cl, 28% polyethylene glycol [PEG] 4000, 20 mM CaCl2 |
