5D93

Oxidoreductase Fragment of Mouse QSOX1 in Complex with a FAb Fragment from a Mouse QSOX1-Specific Antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	65.539, 112.715, 193.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.203 - 2.201
R-factor	0.1771
Rwork	0.174
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d8i 3d85 4q0x 3ab0
RMSD bond length	0.008
RMSD bond angle	1.113
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.088	0.408
Number of reflections	72630
<I/σ(I)>	7.6	2
Completeness [%]	98.9	98.8
Redundancy	4.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	50 mM ammonium sulfate, 0.1 M bis-tris methane buffer, pH 5.5, 22% w/v PEG 3.35 kD