5D7X
Crystal structure of the human BRPF1 bromodomain in complex with XZ08
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.365, 60.365, 63.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.183 - 1.350 |
| R-factor | 0.1682 |
| Rwork | 0.168 |
| R-free | 0.17620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.390 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.311 |
| Number of reflections | 29544 | |
| <I/σ(I)> | 21.6 | 5.7 |
| Completeness [%] | 98.8 | 92.5 |
| Redundancy | 9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M Bis-Tris Propane, pH6.5, 0.2M Sodium Nitrate, 20% PEG3350 |
