5D70
Crystal structure of MOR03929, a neutralizing anti-human GM-CSF antibody Fab fragment in complex with human GM-CSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0015 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 158.235, 158.235, 158.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.890 - 2.060 |
| R-factor | 0.19135 |
| Rwork | 0.190 |
| R-free | 0.22179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5C7X and 2GMF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.890 | 2.240 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.091 | 0.734 |
| Number of reflections | 40747 | |
| <I/σ(I)> | 27.58 | |
| Completeness [%] | 98.9 | 95.9 |
| Redundancy | 21.6 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.75 | 293 | 100 mM Tris, pH 8.75, 200 mM LiSO4, 25-28 % (w/v) PEG 3350, 28mg/mL protein |
