5D6W

Crystal structure of double tudor domain of human lysine demethylase KDM4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	190.644, 190.644, 116.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.084 - 1.992
R-factor	0.1689
Rwork	0.168
R-free	0.19550
RMSD bond length	0.008
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.110
High resolution limit [Å]	1.990	5.930	1.990
Rmerge	0.127	0.062	1.006
Rmeas	0.142	0.070	1.128
Total number of observations	542322
Number of reflections	108314	4109	17438
<I/σ(I)>	9.04	20.12	1.94
Completeness [%]	99.9	99.7	99.4
Redundancy	5.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	Protein Solution (30mg/ml KDM4A DTD, 20mM HEPES pH 7.5, 150mM NaCl and 0.5mM TCEP) mixed in a 1:1 ratio with the well solution (1.0M ammonium tartrate dibasic and 0.1M sodium acetate trihydrate pH4.6) Cryoprotected with additional 20% glycerol