5D6L
beta2AR-T4L - CIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96867 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.260, 170.360, 40.270 |
| Unit cell angles | 90.00, 105.67, 90.00 |
Refinement procedure
| Resolution | 49.760 - 3.200 |
| R-factor | 0.2236 |
| Rwork | 0.222 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rh1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.915 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.760 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.143 | 0.814 |
| Number of reflections | 10858 | |
| <I/σ(I)> | 1.5 | 1.5 |
| Completeness [%] | 94.4 | 96.7 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 30 - 35 % Peg 400, 5 -10 % butanediol, 0.1 - 0.35 mM Na sulphate, 0.1 M Bis-Tris pH 7.0. |
