5D6D

Crystal structure of GASDALIE IgG1 Fc in complex with FcgRIIIa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	74.380, 94.500, 108.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.309 - 3.130
R-factor	0.2731
Rwork	0.271
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sgk
RMSD bond length	0.015
RMSD bond angle	1.459
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.310	3.180
High resolution limit [Å]	3.130	3.130
Rmerge	0.110
Number of reflections	14045
<I/σ(I)>	7.52	0.5
Completeness [%]	100.0	100
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	grown in sitting drop vapor diffusion by mixing equal volumes of protein (10 mg/ml) with a solution containing 0.04 M potassium dihydrogen phosphate and 16% (w/v) PEG 8000 at 293.15 K