5D4Q

Crystal structure of GASDALIE IgG1 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979490
Spacegroup name	P 21 21 21
Unit cell lengths	49.320, 79.130, 137.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.429 - 2.390
R-factor	0.2426
Rwork	0.241
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h3x
RMSD bond length	0.012
RMSD bond angle	1.247
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.430
High resolution limit [Å]	2.390
Rmerge	0.140
Number of reflections	22054
<I/σ(I)>	6.62
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M ammonium formate and 20% (w/v) PEG 3350