5D3Q

Dynamin 1 GTPase-BSE fusion dimer complexed with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 1
Unit cell lengths	44.220, 49.960, 82.520
Unit cell angles	99.40, 87.18, 105.18

Refinement procedure

Resolution	47.626 - 1.700
R-factor	0.1744
Rwork	0.173
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2e
RMSD bond length	0.015
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.626	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.118	0.245
Number of reflections	72213
<I/σ(I)>	10.75	6.7
Completeness [%]	97.6	96.1
Redundancy	4.63	3.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Before crystallization set-ups, GG1 at a concentration of 10 mg/ml was supplemented with 2 mM GDP and 2 mM MgCl2 and incubated for 1 h on ice. GG1GDP was crystallized by hanging drop vapour diffusion mixing 1.5 ul protein solution with 1.5 ul reservoir solution containing 0.1 M Tris (pH 8.0), 26 % PEG 3350 and 0.2 M NaSCN. The drops were equilibrated against 700 ul of reservoir.