5D3E

Crystal structure of human 14-3-3 gamma in complex with CFTR R-domain peptide pS768-pS795

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.978860
Spacegroup name	P 41 21 2
Unit cell lengths	122.450, 122.450, 313.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.300 - 2.750
R-factor	0.2041
Rwork	0.202
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uzd
RMSD bond length	0.014
RMSD bond angle	1.271
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.300	36.300	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.120	0.064	0.639
Rpim	0.034	0.018	0.186
Total number of observations	856403	10889	58320
Number of reflections	62906
<I/σ(I)>	15.6	30.5	4.2
Completeness [%]	100.0	96.3	100
Redundancy	13.6	13.5	12.8
CC(1/2)	0.998	0.998	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, NaCl, DTT, (NH4)2SO4, Poly(acrylic acid sodium salt 2100