5D2D

Crystal structure of human 14-3-3 zeta in complex with CFTR R-domain peptide pS753-pS768

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	77
Detector technology	PIXEL
Collection date	2013-02-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.99983
Spacegroup name	P 41 21 2
Unit cell lengths	112.862, 112.862, 158.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.085 - 2.100
R-factor	0.1945
Rwork	0.193
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.506
Number of reflections	60180
<I/σ(I)>	22.8
Completeness [%]	100.0
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	HEPES, NaCl, DTT, Li2SO4, Glycine, K2HPO4