5D28

Complex of GM-CSF/IL-2 inhibition factor with Granulocyte-macrophage colony-stimulating factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	145.700, 105.600, 73.490
Unit cell angles	90.00, 93.31, 90.00

Refinement procedure

Resolution	44.063 - 2.845
R-factor	0.1928
Rwork	0.191
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d22 2vga 1cq3
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1685)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.840	2.840
Rmerge		1.120
Number of reflections	25798
<I/σ(I)>	20.39	1.77
Completeness [%]	98.2	95.7
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.15 M ammonium sulfate, 0.1 M MES pH 6.0, 24 % PEG 4000