5D22
Structure of ovine granulocyte-macrophage colony-stimulating factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.239 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.100, 77.020, 47.450 |
| Unit cell angles | 90.00, 111.54, 90.00 |
Refinement procedure
| Resolution | 38.510 - 1.994 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gmf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 18252 | |
| <I/σ(I)> | 8.17 | 2.12 |
| Completeness [%] | 96.9 | 94 |
| Redundancy | 3.28 | 3.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M sodium acetate trihydrate 0.1 M TRIS hydrochloride pH 8.5 30% w/v PEG 4000 |
