5D1M
Crystal Structure of UbcH5B in Complex with the RING-U5BR Fragment of AO7 (P199A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.696, 47.415, 67.975 |
Unit cell angles | 90.00, 118.32, 90.00 |
Refinement procedure
Resolution | 30.398 - 1.581 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.22310 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5d1k |
RMSD bond length | 0.009 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.033 | 0.027 | 0.289 |
Number of reflections | 38987 | ||
<I/σ(I)> | 23 | 75.779 | 5.296 |
Completeness [%] | 99.7 | 99.7 | 96.9 |
Redundancy | 6.2 | 6.5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% PEG 10000, 8% Ethylene glycol, 0.1M HEPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |