5D16
Structure of the C-terminal domain of TnsE double mutant - A453V/D523N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-06 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.030, 71.250, 50.430 |
| Unit cell angles | 90.00, 97.28, 90.00 |
Refinement procedure
| Resolution | 29.301 - 1.760 |
| R-factor | 0.1988 |
| Rwork | 0.198 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d17 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.757 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.490 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.030 | 0.973 |
| Number of reflections | 32735 | |
| <I/σ(I)> | 21 | 1.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | PEG 3350, Bis-Tris |
