5D0M

Structure of UbE2D2:RNF165:Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	62.620, 62.620, 82.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.005 - 1.913
R-factor	0.1805
Rwork	0.179
R-free	0.21270
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.310
High resolution limit [Å]	1.910
Number of reflections	24549
<I/σ(I)>	19.3
Completeness [%]	100.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	SPG buffer, PEG 1500