5D0B
Crystal structure of epoxyqueuosine reductase with a tRNA-TYR epoxyqueuosine-modified tRNA stem loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.644, 110.754, 99.223 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 32.049 - 2.645 |
| R-factor | 0.1932 |
| Rwork | 0.192 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d08 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.728 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.740 |
| High resolution limit [Å] | 2.645 | 5.710 | 2.650 |
| Rmerge | 0.154 | 0.102 | 0.337 |
| Rmeas | 0.169 | 0.112 | 0.392 |
| Rpim | 0.067 | 0.045 | 0.194 |
| Total number of observations | 138854 | ||
| Number of reflections | 26382 | ||
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 92.8 | 98.7 | 68.3 |
| Redundancy | 5.3 | 5.6 | 3.3 |
| CC(1/2) | 0.989 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | (0.1 M acetate (pH 4.5), 0.4 M (NH4)H2PO4, 12% (w/v) PEG 3350 |
